@article { author = {Keivanloo, Saeide and Sudagar, Mohammad and Mazandarani, Mohammad}, title = {Evaluating the suitability of cryopreservation solutions for common carp (Cyprinus carpio) embryos stored at -2 °C}, journal = {Iranian Journal of Fisheries Sciences}, volume = {18}, number = {4}, pages = {1035-1045}, year = {2019}, publisher = {Agricultural Research,Education and Extension Organization}, issn = {1562-2916}, eissn = {2322-5696}, doi = {10.22092/ijfs.2019.118495}, abstract = {In the present study, cryopreservation of common carp (Cyprinus carpio) embryos was studied using a stepwise cooling protocol. Embryos at heartbeat stage were selected, placed in 15- mL plastic tubes and stored at -2 °C for 1, 7, 14, and 21 days in 2 different cryopreservation solutions: S1 (methanol + dimethyl sulfoxide + sucrose) and S2 (methanol + propylene glycol + sucrose). Embryo viability was assessed by survival rate, counting live larvae and number of failed eggs under a stereomicroscope.  The results showed that storage of common carp embryos was possible for up to 7 days at -2 °C, while the survival did not occur when storage period were 14 and 21 days. The combination of methanol with propylene glycol (S2) gave higher survival rate after 1 and 7 days stored at -2 °C. Further studies are needed in order to extend the storage time and to improve the survival rate for this species.}, keywords = {Cryopreservation,common carp,Cryoprotectant}, url = {https://jifro.areeo.ac.ir/article_118495.html}, eprint = {https://jifro.areeo.ac.ir/article_118495_e9c2c56158b46be241b8885af56439dc.pdf} }