@article { author = {Mansourghanaei, A. and Khara, H. and Vahabzadeh Roudsari, H. and Pajand, Z. and Ahmadnezhad, M.}, title = {Alterations in Hematological indices, histopathology and p450 gene expression in stellate sturgeon (Acipenser stellatus Pallas, 1811) fingerlings exposed to different salinities levels and ammonia}, journal = {Iranian Journal of Fisheries Sciences}, volume = {21}, number = {6}, pages = {1343-1366}, year = {2022}, publisher = {Agricultural Research,Education and Extension Organization}, issn = {1562-2916}, eissn = {2322-5696}, doi = {10.22092/ijfs.2022.128167}, abstract = {This study was conducted in two stages. At the first stage, the lethal concentrations (LC50 96h) of ammonia in different salinities including, 0ppt  (7.42 mgL-1), 4ppt (8.24 mgL-1), 8ppt  (9.60 mgL-1) and 12 ppt  (10.22 mgL-1) to A. stellatus fingerlings were determined. At the second stage, 240 fish (15.23±2.17 g, mean weight and 17±1.96 cm, total length)  were exposed to half (50%) median lethal concentration (LC50 96h) under the same salinities for four days (8 treatments in triplicates). Maximum Red  blood cells , white blood cell , hematocrit , hemoglobin, mean corpuscular volume , mean corpuscular hemoglobin , mean corpuscular hemoglobin concentration , and differentiation of leucocytes indices were observed in combined treatments (salinity with ammonia) with salinity and ammonia (p≤0.05). Cortisol, glucose, and lactate decreased, meanwhile immunoglobulin M, lysozyme and total immunoglobulin blood serum levels significantly increased with increasing salinity and ammonia in combination treatments (p≤0.05). The activity of superoxide dismutase  and glutathione peroxidase  blood serum increased in combined salinity and ammonia treatments but catalase  decreased significantly (p≤0.05). Alanine aminotransferase  and aspartate aminotransferase enzyme levels increased in combined salinity and ammonia treatments (p≤0.05). But a significant decrease in the levels of alkaline phosphatase  and lactate dehydrogenase  blood serum enzymes was observed (p≤0.05). The highest gill and liver damage was observed in salinity of 12 ppt and also combined treatment of salinity of 12 ppt with ammonia.  The highest hepatic P450 gene expression was observed in the last treatment (p≤0.05). As a result, increasing salinity increasing tolerance, reduced stress and increasing immunity in stellate sturgeon fingerlings at higher concentrations of ammonia. But salinity could not reduce the toxic effects of ammonia on other indicators.}, keywords = {ACIPENSER STELLATUS,Ammonia,Salinity,Hematological indices,Histopathological,Gene expression p450}, url = {https://jifro.areeo.ac.ir/article_128167.html}, eprint = {https://jifro.areeo.ac.ir/article_128167_c53700598ab0d9300209243e0e2a0ca3.pdf} }