Agricultural Research,Education and Extension Organization Iranian Journal of Fisheries Sciences 1562-2916 14 3 2015 07 01 Karyological analysis of Cyprinion macrostomum Heckel, 1843, from Godarkhosh River, Ilam Province, Iran 786 796 114482 10.22092/ijfs.2018.114482 EN M Nasri Y Keivany S Dorafshan Journal Article 2018 01 02 In this study, for the first time in Iran, the karyotype of bigmouth Lotak, Cyprinion macrostomum Heckel, 1843, was investigated through examining metaphase chromosomes of seven fish with mean weight 30±5g caught by electrofishing from Godarkhosh River in Ilam Province. To stimulate cell divisions, fish were injected intraperitoneally two times by phytohemagglutinin (PHA). The cell divisions were arrested in metaphase stage by intraperitoneal injection of colchicine. Well-separated cells were obtained from kidney and gill filament and chromosome spreads were prepared and stained with giemsa. Karyotype was obtained as 2n=50. The karyotype consisted of 5 metacentric, 12 submetacentric and 8 telocentric chromosome pairs. Centromeric index, arm ratio and Fundamental Number (FN) were determined as 0-50, 1-∞, and 84, respectively. https://jifro.areeo.ac.ir/article_114482_bfd09eb81ae536f160099d7b6f29b3e5.pdf