Agricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291621520220901Hatchability improvement in ephippia Moina macrocopa through substitution of rice bran suspension with fishmeal suspension1113112412767410.22092/ijfs.2022.350947.0ENAhmad Shofy Mubarak ShofyAirlangga UniversityDedi Jusadi DediBogor Agricultural UniversityMuhammad Zairin Junior ZairinBogor Agricultural UniversityMuhammad Agus Suprayudi AgusBogor Agricultural UniversityJournal Article20200304Ephippia <em>Moina macrocopa</em><strong> </strong> hatching relates to the quality of nutrients received by the parent. Increasing the quality of the parent feed can improve the quality of the nutrient in eggs (ephippium) and can increase hatchability. This study was conducted to examine the addition of fatty acids in the feed through replacing rice bran suspension with fishmeal suspension on the quality and quantity of ephippia <em>M. macrocopa</em>. The treatment in this research was a substitution of rice bran suspension with fishmeal suspension by 0, 15, 30, and 45%. This study indicated that substitution of rice bran suspension with fishmeal suspension of 30% and 45% in Moina culture produced the same ephippia production but with a higher degree of hatching. Moreover, the substitution of rice bran suspension with fishmeal suspension by 30% increased the concentration of n-3 fatty acids (1.15%) in the egg of ephippia <em>M. macrocopa</em>.https://jifro.areeo.ac.ir/article_127674_7aab01ccfc9a0a2299815a5b8213ecd9.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291621520220901Effects of dietary selenium and zinc nanoparticles on growth performance, immune responses, and antioxidant enzymes activities of white leg shrimp (Litopenaeus vannamei)1125114012767710.22092/ijfs.2022.127677ENM. KaramzadehDepartment of Fisheries, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas, IranM. YahyaviDepartment of Fisheries, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas, IranA.R. SalarzadehDepartment of Fisheries, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas, IranD. Nokhbe ZarDepartment of Fisheries, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas, IranJournal Article20221004In the present study selenium nanoparticles (Se-NPs) and zinc nanoparticles (Zn-NPs) included in the diets of white shrimp at 0 mg (control), 0.3 mg Se-NPs/ kg feed (T1), 0.15 mg Se-NPs+15 mg Zn-NPs/kg feed (T2) and 30 mg Zn-NPs/kg feed (T3). After 8 weeks, shrimps fed both Se-NPs and Zn-NPs showed higher (<em>p</em><0.05) final body weigh, weigh gain, and SGR than shrimp fed the control diet. On the other hand, the FCR was decreased by Se-NPs and Zn-NPs when compared to the control whereas both Se-NPs+Zn-NPs revealed lower FCR than the other groups (<em>p</em><0.05). Shrimp fed Se-NPs or Se-NPs +Zn-NPs revealed higher survival rate than shrimp fed the control and Zn-NPs while shrimp fed Zn-NPs showed higher survival rate than shrimp fed the control (<em>p</em><0.05). The total haemocyte count, large granular cells, semi granular cells, and semi granular cells revealed higher counts in shrimps fed Se-NPs and Zn-NPs than the control (<em>p</em><0.05). Shrimp fed Se-NPs or Se-NPs+ Zn-NPs revealed higher catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidas (GPX) activities than shrimp fed the control and Zn-NPs while shrimp fed Zn-NPs showed higher catalase activity than shrimp fed the control (<em>P</em><0.05). The total protein, lysozyme, and phenoloxidase activities increased in shrimp fed Se-NPs or/and Zn-NPs with the highest being in shrimp fed both in shrimp fed Se-NPs and Zn-NPs followed by those fed Se-NPs then Zn-NPs when compare to the control (<em>p</em><0.05). In conclusion, the co supplementation of Se-NPs and Zn-NPs showed higher performances, antioxidant status, and immune responses in white shrimp than the individual supplementation of Se-NPs or Zn-NPs.https://jifro.areeo.ac.ir/article_127677_89a06e15c058d6ee8c45942a246024c8.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291621520220901Extraction and investigation of biological activities of dioctyl phthalate and dibutyl phthalate from marine sponge Haliclona (Soestella) caerulea Larak Island, Persian Gulf1141115512771010.22092/ijfs.2022.127710ENE. ShafeianP. Ghavam MostafaviDepartment of Marine Science, Faculty of Natural Resources and Environment, Science and Research Branch, Islamic Azad University, Tehran, Iran.M. Moridi FarimaniA. Mashinchian MoradiM. NazemiJournal Article20221009Marines are unique resource that provides a diverse array of natural products, primarily from invertebrates such as sponge. As infectious diseases evolve and develop resistance to existing pharmaceuticals, these ecosystems provide novel leads against microbial, cancer, and viral diseases. The purpose of this study is to investigate antimicrobial and antifungal activities of derivatives of phthalates extracted from <em>Haliclona</em> (<em>Soestella</em>)<em> </em><em>caerulea</em> on some pathogenic organisms. Sponge samples from Larak Island were collected by divers at a depth of 10 meters. After that, marine sponge extract collection was done by chloroform-methanol extraction as a solvent and through chromatography column and nuclear magnetic resonance (NMR) spectroscopy on the sponge sample, components of dioctyl phthalate (DOTP) and dibutyl phthalate (DBP) were purified and identified. Anti-microbial and antifungal activity of extracted components were investigated by means of bacterial broth dilution method and microdilution broth method and minimum inhibitor concentration (MIC), minimum bactericide concentration (MBC), and minimum fungal concentration (MFC) were determined. The results indicated that DOTP of <em>H. </em>(<em>Soestella</em>)<em> caerulea</em> was active on <em>Bacillus subtilis</em>, <em>Bacillus cereus</em> bacteria. MBC values of DOTP were determined as 2000 μg/m. DBP of <em>H. </em>(<em>Soestella</em>)<em> caerulea</em> was active on gram positive bacteria. MBC values of DOTP were determined as 1000 μg/m for <em>B. cereus</em> and <em>Staphylococcus aureus</em> and 500 μg/mL for <em>B. subtilis</em>. MFC values of DOTP and DBP were determined as 1000 μg/m and 2000 for <em>Candida albicans</em>. Hence it is assumed that the sponge <em>Haliclona </em>(<em>Soestella</em>)<em> </em><em>caerulea</em> exhibited high antimicrobial activity.https://jifro.areeo.ac.ir/article_127710_f822d8da664e3781aff4d0656a620dc3.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291621520220901Life stages and seasonal variances of salinity tolerance of Triplophysa yarkandensis (Day,1877) in Tarim River1156116512771110.22092/ijfs.2022.127711ENZhichao WANGCollege of Life ScienceXiaolei WANGdepartmentJunyuan WUCollege of Life ScienceDaoquan RENfishery dCuifang YANGCollege of Information EngineeringJournal Article20151129 <br /> <br /> <br /> https://jifro.areeo.ac.ir/article_127711_14f60d9770c033ffad8a07692d6c8771.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291621520220901Effect of extracellular proteins (ECP) on protective efficacy of Yersiniosis vaccine in juvenile rainbow trout (Oncorhynchus mykiss)1166117912776510.22092/ijfs.2022.127765ENZahra Tulaby DezfulyPh.D. student of Aquatic Science, Faculty of Veterinary Medicine, ShahidChamran University of Ahvaz, Ahvaz, IranMojtaba AlishahiProfessor of Department of Clinical SciencesMasoud GhorbanpoorProfessor of Department of microbiologyMohammad Reza TabandehAssociate professor of Biochemistry and Molecular DepartmentMehrzad MesbahProfessor of Department of Clinical SciencesJournal Article20190204Yersiniosis is the second important bacterial infections in coldwater fish culture with significant mortalities and economical losses in the Iranian fish farms. In the present study, the effect of extracellular proteins (ECP) on protective immunity<strong> </strong>of <em>Y. ruckeri</em> vaccine was evaluated in juvenile rainbow trout (7±1.2 g). For this purpose, 540 specimens of juvenile rainbow trout were randomly divided into 6 groups each in triplicates. Group 1 (G<sub>1</sub>) and Group 2 (G<sub>2</sub>) were orally administrated with formalin killed cells (FKC) and FKC+ECP, respectively. Groups 3 to 5 received ECP, FKC, ECP+FKC via intraperitoneal route, respectively. Group 6 received phosphate buffer saline as the control group. The humoral antibody responses to bacterial antigens were monitored by ELISA. LD<sub>50</sub> of <em>Y. ruckeri</em> was determined used probit method sixty days after vaccination. Then, fish in each treatment were challenged intraperitoneally (I.P) with LD<sub>50</sub> of <em>Y. ruckeri</em> virulent registered strain in Iran (KCW 291153). The ELISA results indicated that ECP could increase the serum ELISA antibody titer as the humoral immune response, but ECP with the FKC could increase antibody levels significantly in serum and intestine mucus. Also survival rates in G<sub>1 </sub>to G<sub>6</sub> were 25, 31.25, 37.5, 56.25, 87.5 and 15 percent, respectively. Among I.P immunized fish the survival rate in G<sub>5</sub> was significantly higher than the other groups. Although in orally vaccinated fish with FKC (G1) survival rate did not show significant difference with the control group, the FKC /ECP group (G<sub>2</sub>) showed a significant increase compared to control group (<em>p</em>=0.039). It was concluded that supplementing FKC with ECP increased protective immunity of Yersiniosis vaccine principally in I.P. route. Given the great benefits of the oral vaccine, whole cell/ECP can be considered as a protective antigen to design potential vaccines against this pathogenic micro-organism.https://jifro.areeo.ac.ir/article_127765_1f6059403401a8dfb0de26d3cd5bb59d.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291621520220901Bioactive peptides from the Pacific white-leg shrimp (Litopenaeus vannamei) induce apoptosis and anticancer activities in HCT-116 colon cancer cell line1180119112785210.22092/ijfs.2022.127852ENN. AzadiDepartment of Biology, Faculty of Sciences, Urmia University, Urmia, IranE. H. NajdegeramiDepartment of Biology, Faculty of Sciences, Urmia University, Urmia, IranM. ImaniDepartment of Basic Science, Faculty of Veterinary Medicine, Urmia University, Urmia, IranM. NikooDepartment of Pathobiology and Quality Control, Artemia & Aquaculture Research Institute, Urmia University, Urmia, IranJournal Article20221030In this study, the effects of white-leg shrimp hydrolysate protein extracted in a progressive rise in temperature (40-60°C) (Gradual) and without centrifugation (Mix) on the colon cancer cells were determined. Both bioactive peptides were prepared using enzymatic hydrolysis with alcalase. The cytotoxic effect on HCT-116 cell line was evaluated using the Neutral red and MTT assay. <em>In-vitro</em>, antioxidant activity was performed using DPPH, TAC, and nitric oxide assays. Apoptosis with acridine orange/etho bromide and redox changes was evaluated in the cell lines.The results of toxicity assays showed that the survival rate of the cells were decreased by increasing the concentrations of the peptides (0.3, 0.6, 1.2, and 2.4 mg/mL). Based on the results of antioxidant activity (TAC, DPPH, No), the Gradual peptides had significantly higher antioxidant activity than Mix peptides (<em>p<</em>0.05). In addition, the Gradual peptide increased the concentration of nitric oxide compared to the control and Mix groups (<em>p</em><0.05). Results also showed that the Gradual peptides reduced the secretion of catalase and GSH enzymes in the cancer cells (<em>p</em><0.05). Both types of peptides increased apoptosis in the cancerous cell line and a higher value was observed in bioactive peptides treatments The results of the present study showed that the Pacific white shrimp hydrolysates protein obtained in a progressive rise in temperature showed anti-cancer activities against colon HCT-116cancer cell line.https://jifro.areeo.ac.ir/article_127852_d03158153df6865cbcdb89bfe7a5b5c7.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291621520220901Utilization of mallow, Malva parviflora, leaves meal for feeding sailfin molly fish Poecilia latipinna (Lesueur, 1821)1192120312795010.22092/ijfs.2022.127950ENR.A. Al-TameemiDepartment of Fisheries and Marine Resources, College of Agriculture, University of Basrah, Basrah, IraqR.M. Sayed-LafiDepartment of Fisheries and Marine Resources, College of Agriculture, University of Basrah, Basrah, IraqS.M. NajimDepartment of Fisheries and Marine Resources, College of Agriculture, University of Basrah, Basrah, IraqJournal Article20221109The present study was carried out to investigate the effect of replacing some conventional feedstuffs (barley and yellow corn) with mallow, <em>Malva parviflora,</em> leaves meal on the growth and feeding efficiency of sailfin molly, <em>Poecilia latipinna</em>. Five experimental diets containing 0, 5, 10, 15, and 20% of mallow leaves meal M<sub>0</sub>, M<sub>5</sub>, M<sub>10</sub>, M<sub>15</sub>, and M<sub>20</sub> respectively, were prepared by replacing barley and yellow corn. A total of 90 fish (2.55±0.17 g) were used, distributed equally into fifteen plastic tanks at a rate of 6 fish per tank. The experiment lasted for 60 days, during which fish were fed the experimental diets to satiation twice daily. Results exhibited a gradual significant (<em>p</em><0.05) increase in the feed intake FI (r=0.935) of the fishes with increasing dietary mallow leave replacement. There were no significant differences (<em>p</em>>0.05) in specific growth rate (SGR), feed conversion ratio (FCR), and protein efficiency ratio of sailfin molly fed control (M<sub>0</sub>) diet compared to fish fed on M<sub>5</sub> and M<sub>10 </sub>diets at the end of the experiment. Increasing the involvement of mallow in M<sub>15</sub> and M<sub>20</sub> diets led to inhibited SGR, FCR, and PER significantly (<em>p</em><0.05) compared to the control diet. In conclusion, using mallow dried meal in the diets at a 10% level was proved to be more suitable with no noticeable adverse effects on the growth and feeding efficiency of experimental fish.https://jifro.areeo.ac.ir/article_127950_ffd18cdccab318b742842b766267cec9.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291621520220901A comparative study on some properties and antioxidant activity of Hypophthalmichthys molitrix (Valenciennes, 1844) protein hydrolysates produced by different hydrolysis methods1204121912795110.22092/ijfs.2022.127951ENAtena Hasanzati RostamiDepartment of Fisheries Science, College of Agriculture, Tehran Science and Research Branch, Islamic Azad University, Tehran, IranAli MotamedzadeganDepartment of Food Science and Technology, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Sari, IranSeyed Ebrahim HosseiniDepartment of Food Science and Technology, College of Agriculture, Tehran Science and Research Branch, Islamic Azad University, Tehran, IranMasoud RezaeiDepartment of Fisheries, Faculty of Marine Sciences, Tarbiat Modares University, P.O. Box 46414-356, Noor, IranAbolghasem KamaliDepartment of Fisheries Science, College of Agriculture, Tehran Science and Research Branch, Islamic Azad University, Tehran, IranJournal Article20171231Some functional, physicochemical and antioxidant properties of<em> Hypophthalmichthys molitrix</em> protein hydrolysates produced by alcalase (SCPH-alcalase), acid (SCPH-acidic) and alkaline (SCPH-alkaline) were investigated. No significant difference was observed for degree of hydrolysis (DH), L*, a* and b* values among all types of hydrolysate samples (<em>p</em>>0.05). The highest protein recovery, protein and essential amino acids content were observed in SCPH-alcalase. All protein hydrolysates displayed antioxidant activities, that enhanced by increasing the hydrolysates concentration. The chemical hydrolysates had the highest DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging activity, while SCPH-alcalase showed the highest ABTS (2, 2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)) radical-scavenging activity and FRAP (Ferric reducing antioxidant power) (<em>p</em><0.05). The highest solubility and the lowest fat adsorption were observed with the SCPH-alcalase (<em>p</em><0.05). Increased numbers of hydrophilic groups of SCPH-alcalase are supported by FTIR spectra. The results showed the significant effect of different hydrolysis conditions on hydrolysates properties and indicated alcalase enzyme might be more suitable to produce hydrolysates with desirable functional, nutrition and antioxidative properties.https://jifro.areeo.ac.ir/article_127951_2e57f517e6d17b6d56fdc9ddbd560b19.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291621520220901Effects of dietary protein, lipid and carbohydrate levels on hematological parameters, intestinal histoarchitecture and digestive enzymes activities in orange-spotted grouper (Epinephelus coioides) juveniles1222124012798110.22092/ijfs.2022.127981ENF. KianradDepartment of Fisheries, Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr, IranA. OujifardDepartment of Fisheries, Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr, IranE. SotoudehDepartment of Fisheries, Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr, IranM. Nafisi BahabadiDepartment of Fisheries, Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr, IranV. MorshediDepartment of Fisheries, Persian Gulf University, Bushehr, IranM. Torfi MozanzadehAquaculture Research Center -South of Iran, Iranian Fisheries Science Research Institute, Agriculture Research, Education and Extension, Ahwaz, IranJournal Article20221115A 56-day research was conducted to assess the influence of dietary protein content and carbohydrate: lipid ratio on growth performance, hematological parameters, intestinal histoarchitecture and digestive enzymes activities of orange-spotted grouper (<em>Epinephelus coioides</em>) juveniles (13.7 g). Four experimental diets were designed with various protein, lipid and carbohydrate levels namely LP-LCL (low protein 40%−low carbohydrate: lipid ratio=1.4), HP-LCL (high protein 48%−low carbohydrate: lipid ratio=1.4), LP-HCL (low protein 40%−high carbohydrate: lipid ratio=2.9) and HP-HCL (high protein 48%−high carbohydrate: lipid ratio=2.9). Fish were stocked into twelve 300-L cylindrical polyethylene tanks (15 fish in each tank) and fed with experimental diets. The low carbohydrate: lipid ratio diets improved growth rate of the fish. The lipid content of the whole body increased with decreasing dietary carbohydrate to lipid ratio. The intestinal folds’ length and thickness significantly increased in the low carbohydrate: lipid ratio treatments. The intestinal total alkaline protease activity elevated by increasing dietary protein content, meanwhile the activities of lipase and amylase increased with increasing dietary lipid and carbohydrate levels, respectively. Blood hematocrit decreased but hemoglobin increased by increasing dietary carbohydrate to lipid ratio. According to the results dietary lipid is more preferable than carbohydrate as an energy source for orange-spotted grouper juveniles and induces protein sparing effect in a low protein content diet.https://jifro.areeo.ac.ir/article_127981_020b228538306223ebdc826cb800058e.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291621520220901Time-dependent changes of alkaline, neutral, and acid protease activities, molecular weight distributions, and proximate compositions of live feeds1241126612800810.22092/ijfs.2022.350968.0ENG. DikenIsparta University of Applied Sciences Faculty of E?irdir FisheriesJournal Article20200326In this study, alkaline, neutral, and acid enzyme activities, molecular weight distributions, and proximate compositions of live feeds were determined in a time-dependent manner. Samples were taken from three different live feeds and <em>Artemia</em> cysts. R refers to rotifer, A-0 refers to non-enriched <em>Artemia</em> / <em>A</em>. nauplii, and A-1 refers to enriched <em>Artemia</em> / <em>A</em>. metanauplii. Sampling was done at 0<sup>th</sup>, 6<sup>th</sup>, 12<sup>th</sup>, and 18<sup>th</sup> h (T) and before enrichment as R (R-0) and A1 (A1-0). Depending on time, while enriched rotifers have high acid enzyme values at pH 3 and 4, neutral and alkaline enzyme values of enriched <em>Artemia</em>s were higher (<em>p<</em>0.05). Time-dependent decreases were detected in the enzyme activities of enriched <em>Artemia</em> and rotifers. Live feeds enriched for up to 12 hours should be used with a molecular weight fraction of 2,532 Da≥ (<em>p<</em>0.05). When marine finfish such as European sea bass and gilthead sea bream are started feeding, their digestive enzymes have alkaline and then acid characteristics. On the other hand, in this study, it was determined that rotifer enzymes had higher acid protease activities and <em>A</em>. metanauplii had higher alkaline protease enzyme activities. Considering this situation, <em>A</em>. nauplii should be given together with rotifers and <em>A</em>. metanuplii should be started as soon as possible. In addition, considering the enzyme activity values of the rotifers used as the first feed, the use of rotifers that are not enriched in terms of the contribution of live feeds to the larval digestive system should also be taken into account.https://jifro.areeo.ac.ir/article_128008_5a053bc36ef69074bea8b08d0d9d22ec.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291621520220901Preparation and biodistribution study of 67Ga-rosemary (Salvia rosmarinus Spenn.) extract nanoparticles and its SPECT imaging in healthy rainbow trout (Oncorhynchus mykiss)1267127712801010.22092/ijfs.2022.128010ENN. Damavandi KamaliDepartment of Seafood Science and Technology, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resource, Gorgan, Iran.S. RajabifarNuclear Science and Technology Research Institute, Tehran, Iran.A.R. AlishahiDepartment of Seafood Science and Technology, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resource, Gorgan, Iran.M. HeidariehNuclear Science and Technology Research Institute, Tehran, Iran.S.M. OjaghDepartment of Seafood Science and Technology, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resource, Gorgan, Iran.S.H. MirsadeghiDepartment of Seafood Science and Technology, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resource, Gorgan, Iran.Journal Article20221121The aim of this study was to evaluate the labeling of rosemary extract nanoparticles (RE-NPs) using <sup>67</sup>Ga<sup> </sup>radioisotope, and their biodistribution in healthy rainbow trout (<em>Oncorhynchus mykiss</em>) tissues. RE-NPs were prepared by gamma irradiation (irradiated at a dose of 30 kGy of gamma-ray by <sup>60</sup>Co (PX-30 IssIedovapel, Russia, dose rate of 0.02 Gy sec<sup>-1</sup>) and ultra-sonication methods at two concentrations of 0.5% and 1% and labeled by <sup>67</sup>Ga radioisotope. Quality control studies were done using the RTLC method. The radiolabeling RE-NPs at 1% concentration showed 97% efficiency using a mixture of sodium acetate and acetic acid buffer at pH=6.5 after 30 min at room temperature. In the biodistribution study, 54 healthy fish were randomly distributed into two treatment groups (n=18 fish/treatment group) and one group served as healthy control. Fish received 3.7-7.4 MBq (300 µL) of radiolabeled RE-NPs at 1% concentration and free <sup>67</sup>GaCl<sub>3</sub>, intraperitoneally. The <sup>67</sup>Ga-RE-NPs single-photon emission computed tomography (SPECT) demonstrated the highest tracer accumulation in the kidney and the least uptake in the brain at 24 h after injection of <sup>67</sup>Ga-RE-NPs. However, 48 h after injection of <sup>67</sup>Ga-RE-NPs, the uptake was negligible in all organs as compared to free <sup>67</sup>GaCl<sub>3</sub>. The obtained results revealed that administration of <sup>67</sup>Ga-RE-NPs, as a natural tracer, by IP injection can be a good imaging method to visualize and understand the whole-body, especially the kidney and spleen tissues distribution and pharmacokinetics in healthy rainbow trout.https://jifro.areeo.ac.ir/article_128010_9ea3fa1a1e04c88c5c1c7ae960aeeaca.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291621520220901Bioinformatics and mRNA expression of catalase gene and determination of catalase enzyme activity in zebrafish (Danio rerio) exposed to the herbicide paraquat1278129712801110.22092/ijfs.2022.128011ENM. BayırDepartment of Agricultural Biotechnology, Faculty of Agriculture, Atatürk University, 25240, Erzurum, Turkey.E.C. ÇapanDepartment of Agricultural Biotechnology, Faculty of Agriculture, Atatürk University, 25240, Erzurum, Turkey.S. KeşanDepartment of Agricultural Biotechnology, Faculty of Agriculture, Atatürk University, 25240, Erzurum, Turkey.Journal Article20221121Effects of two different doses, treatment I (0.30 ppm) and treatment II (0.15 ppm) of paraquat, were investigated on mRNA transcript of the <em>catalase </em>gene (<em>cat</em>) and CAT activity in zebrafish. We determined tissue-specific distribution of zebrafish <em>cat</em> and bioinformatics analysis. Muscle, liver, intestine, heart, kidney, brain, eye, gills, swim bladder, skin, ovary and testis tissue samples from three female and three male fish were dissected to detect tissue-spesific distrubition while liver and gill tissues were dissected to evaluate the effect of acute stress in zebrafish. The steady-state level of zebrafish <em>cat </em>transcript in gill (311±11.32) was lower than liver (595.53±14.41) but higher than muscle (132.89±9.21), testis (120.03±6.99), kidney (118.96±8.87), ovary (104.31±8.22), intestine (37.28±3.11), brain (19.17±2.01), eye (18.30±2.10), heart (15.79±2.01), spleen (7.7±0.91), swim bladder (6.97±0.21), and skin (4.59±0.31). Expression of <em>cat</em> was significantly decreased in liver and gill tissues after exposure to two concentrations of paraquat (<em>p</em><0.05). Measurement of enzyme activities in zebrafish, exposed to 0.30 and 0.15 ppm of paraquat for 72 h duration, showed significant (<em>p</em><0.05) variations in CAT activity compared to that of the control. Different steady-state levels among tissues in zebrafish could be explained by duplicated genes in teleost fish that arose through teleost specific-whole genome duplication exhibiting a dissimilar tissue distribution.https://jifro.areeo.ac.ir/article_128011_5b52ca787450d416e44f0fcebef6b14c.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291621520220901Identification and distribution of Gastropods in the east coasts of Qeshm Island (the Persian Gulf, Iran)1298131512813310.22092/ijfs.2022.128133ENN. GholinezhadFaculty of Marine Science and Technology, North Tehran Branch, Islamic Azad University, Tehran, IranA. Ashja ArdalanFaculty of Marine Science and Technology, North Tehran Branch, Islamic Azad University, Tehran, IranM. MalekSchool of Biology, College of Science, University of Tehran, Tehran, IranJournal Article20221217Gastropods are considered as the main molluscs of rocky shores and often show marked zonation patterns both horizontally and vertically. Biodiversity and distribution of gastropods were investigated at 8 stations on rocky shores of Qeshm Island during the coldest and warmest months January and August, respectively, in 2018. In the present study, 35 gastropod species were identified. The highest frequency of gastropod (550±345) was observed at S2 station (Tula 2) and the lowest one was observed at S8 station (Naz Island) (273±83.70). <em>Planaxis sulcatus</em> was found as the dominant species at all stations (%55.76). The results of the 3-way permutational (MANOVA) test showed that the species’ structures of the gastropod community are different at various stations and during sampling months. The results of the nMDS test confirmed these differences in the structure and species composition of the gastropod population during two months. The highest diversity (Shannon index: 3.13) and species richness (Margalef index: 4.53) was observed at the S2 station and the lowest diversity (Shannon index: 2.73, Margalef index: 3.03) was observed at the S8station. According to the results of the <em>SIMPER </em>test, the highest percentage of participation in Bray-Curtis dissimilarity between the two months in summer and winter and at different sampling stations in terms of species density was related to <em>Planaxis sulcatus</em>. The structure of gastropod population at the studied stations can be influenced by the factor of seasonal changes.https://jifro.areeo.ac.ir/article_128133_0706dbb6547dffbe3f97434d7798bdc3.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291621520220901Molecular identification, phylogenetic analysis and histopathological evaluation of gill fungal infection in some ornamental fish: First report and new species1316133412816110.22092/ijfs.2022.128161ENM. Ebrahimi JafariDepartment of Pathobiology, Faculty of Veterinary Specialized Sciences, Science and Research Branch, Islamic Azad University, Tehran, IranM. BayatDepartment of Pathobiology, Faculty of Veterinary Specialized Sciences, Science and Research Branch, Islamic Azad University, Tehran, IranA. Haghighi Khiabanian AslDepartment of Pathobiology, Faculty of Veterinary Specialized Sciences, Science and Research Branch, Islamic Azad University, Tehran, IranS.J. Hashemi HazavehDepartment of Medical Parasitology and Mycology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, IranJournal Article20221218Among infectious pathogens, fungal and fungal like organisms cause the remarkable social and economic impacts on ornamental farmers. Some freshwater ornamental fishes including the cardinal <em>Carassius auratus,</em> Green Terror Cichlids<em> (Andinoacara rivulatus),</em> the guppy (<em>Poecilia reticulata</em>), the Koi (<em>Cyprinus rubrofuscus</em>) with typical signs of severe respiratory symptoms were prepared from a large commercial breeding centers ornamental fish shops in Tehran, Iran. The gill samples were taken and laboratory evaluations carried out. Accordingly, Culturing of fungal isolates, sequencing and molecular identification, pathogenicity as well as gross and microscopic evaluations were also carried out. Four isolated with NCBI-accession numbers of OL891804, OL912959, OL990026 and OL912961, respectivelyas Fungal sp., <em>Penicillium</em> sp., Fungal sp. and <em>Candida</em> sp. were reported and registered in Genebank. Eventually, the Ornamental fish diseases of Tehran ornamental fish centers where a few fish including the cardinal (<em>C. auratus</em>)<em>,</em> Green Terror Cichlids<em> (A. rivulatus),</em> the guppy (<em>P. reticulata</em>) and the Koi (<em>C. rubrofuscus</em>), were suffering from gill disorders had been infected with <em>Penicillium crustosum</em><em>, Alternaria alternata, Filobasidium </em>like organism<em>, Candida zeylanoides, </em>respectively<em>.</em>https://jifro.areeo.ac.ir/article_128161_f83d09c71efecd05700825bb5edad58b.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291621520220901First record of the non-native species Beroe ovata Mayer 1912 (Ctenophora: Nuda) in the Caspian Sea1335134212816210.22092/ijfs.2022.128162ENH. RoohiCaspian Sea Ecology Research Center (CSERC), Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Iran.Journal Article20221218During the late 1980s, carnivorous ctenophore <em>Mnemiopsis leidyi</em>, along with the other invaders, had been transported to the Black Sea from the coasts of eastern Atlantic, which resulted in ecosystem damage for the region (Vinogradov <em>et al</em>., 1989; Kideys, 2002a; Ghabooli <em>et al</em>., 2011). One of the major factors, resulted in the rapid expansion of the <em>M. leidyi</em> population during its initial invasion into the Black Sea, was the absence of a specialised predator (Kideys and Shiganova, 2001; Kideys <em>et al</em>., 2004a, 2004b, 2005, 2008; Roohi <em>et al</em>., 2008). The Black Sea, besides being a recipient area, also serves as a donor area for some invasive species that then spread further to the Sea of Azov, the Sea of Marmara, and the eastern Mediterranean Sea and, in some cases, to the Caspian Sea (Shiganova, 2010).https://jifro.areeo.ac.ir/article_128162_692baa92a34de906841c1a43eccb600a.pdf