Isolation and characterization of microsatellite loci in the Persian sturgeon (Acipenser persicus, Borodine, 1897) and cross-species amplification in four commercial sturgeons from the Caspian Sea



 In order
to have a sustainable management on Persian sturgeon as a highly commercial
species in the South Caspian Sea, we need to identify its population structure
and the level as well as its conservation status in their natural habitat. To
develop a conservation program for this all Caspian Sea' sturgeon species it requires
knowledge of its genetic diversity using reliable molecular marker to study
population genetic structure. For these purposes, an enriched library was prepared
based on a modified biotin-capture method. Approximately 1800 positive clones were
screened for microsatellites in an Acipenser
persicus genomic library. Of these 350 positively hybridizing clones were
sequenced, and 81 clones were identified as having microsatellites with
adequate flanking regions. We developed and tested 68 microsatellite primer pairs
for Persian sturgeon. Out of 68 primer pairs developed, 11 pairs resulted in
poor or no amplification, 13 were ambiguous, 6 were monomorphic, 20 were
tetrasomic and 18 were octosomic in Persian sturgeon. While none of the markers
showed disomic inheritance in Persian sturgeon and Russian sturgeon (A. gueldenstaedtii).
Several of the markers appeared useful for studies stellate sturgeon (A.
stellatus), ship sturgeon (A.nudiventris) and beluga (Huso huso). Nearly
all the polymorphic pattern for ship, stellate and beluga displayed the simple
banding patterns characteristic of disomic loci, while those for Russian
sturgeon displayed banding patterns characteristic of tetraploid or higher
polyploid levels. These markers may prove useful in a variety
of future sturgeon population genetic studies in the Caspian