Sperm quality, egg size, fecundity and their relationships with fertilization rate of grass carp (Ctenopharyngodon idella)



were collected by abdominal massage from 15 anesthesized male and female grass
carp. In collected sperm samples the following mean spermatological properties
were determined: sperm volume 15.64 ± 5.39 mL, sperm motility 81.20 ± 7.61%, motility
duration 62.06 ± 19.40s, density 17.88±5.48 x109 mL-1,
total density 428.90±302.05 x109, and pH 7.25±0.81. Also egg size
(mm) and fecundity (egg/fish) were determined as 1.04±0.028
and 417867 ± 36.274 respectively in female grass carp. The fertilization
capacity of sperm was tested with the same egg pool. The insemination dosage
was 2x105 spz/egg for each fertilization experiment. Fertilization
rate was determined as mean 79.3±2.95% and positively correlated with sperm
motility (r=0.932, p < 0.01), egg size (r=0.513,
p>0.05) and fecundity (r=0.539, p>0.05).