A putative probiotic isolated from hatchery reared juvenile Penaeus monodon



 Probiotic is
considered as a remedy to solve disease problem in shrimp aquaculture. Shrimp
body and environment bacterial flora are recommended as reliable source for
extracting of beneficial bacteria. Therefore, 5000 pieces of post larvae of Penaeus
monodon were distributed equally in 4 tanks in probiotic and antibiotic
free condition up to juvenile. Then microbiological samples from digestive system,
muscles, body surface, rearing water and tank sediment were inoculated onto the
selective media plates. Incident of total bacteria, Vibrio, Pseudomonas
and Entrobacteriaceae were evaluated in different body parts and rearing
environment of shrimp. The highest bacteria count for total bacteria, Vibrio,
Pseudomonas was occurred in sediment followed by digestive system. No Entrobacteriaceae
was observed from all samples. Isolated bacteria were identified and placed in
7 genera consisted of Vibrio, Shewanella, Burkholderia,
Clavibacter, Staphylococcus, Brevibacterium,
Corynebacterium, while one genus remained unidentified. Genus Shewanella
exhibited highest frequent bacteria in digestive system of P. monodon which
may be indicated its ability to colonization in digestive system. Shewanella
algae was selected from whole isolated bacteria as candidate probiotic via
well plate diffusion technique and cross streak antagonistic test against Vibrio
harveyi, V. parahaemolyticus and V. alginolyticus. It was
able to digest lipid and protein in tributyrin agar and skim milk agar.