Chitinase Isolated from Water and Soil Bacteria in Shrimp farming Ponds

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Abstract

 Chitinases have received
attention because of their wide applications in the medicine, biotechnology,
agriculture, waste management and industrial applications such as food quality
enhancer and biopesticide. Excessive use of insecticides has led to several
problems related to pollution and environmental degradation. In this study, isolation and identification of native bacterial
strains with chitin hydrolysis activity, took place from water and soil of shrimp
culture ponds in Bushehr and Abadan.
To investigate the capacity of our chitinase for using in insecticide,
biochemical properties of selected chitinase obtained in this research were
compared to that of produced by Bacillus cereus p-1, isolated
from an insecticide tablet. In this
research, three mesophilic strains containing: Serratia marcescens B4A, Citrobacter
freundii B1A and Bacillus cereus B3R were isolated. Results showed a 1600 bp band corresponding to
chitinase gene. The similarity between temperature and pH profile and stability of
chitinase was extracted from native bacteria and ones was obtained from Bacillus
cereus p-1 implied that chitinase extracted from Serratia
marcescens B4A has potential application in industry.  

Keywords

Iranian Journal of Fisheries Sciences
Volume 11, Issue 4
October 2012
Pages 911-925

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