The aim of this research was to study the occurrence of Vibrio spp., Aeromonas hydrophila, Escherichia coli and Campylobacter spp. in crayfish from Azerbaijan Province using culture method and PCR assay. A total of 55 isolates were collected from 97 studied samples. Vibrio spp., A. hydrophila, E. coli and Campylobacter spp. were detected in 26 samples (26.8%), 12 samples (12.3%), 15 samples (15.46%) and 2 samples (2.06%), respectively. Among Vibrio isolates, Vibrio vulnificus (11.3%) was the species most frequently detected followed by V. harveyi (7.2%), V. alginolyticus (2.06%) and V. mimicus (1.03%). The results of this study indicated that crayfish from the studied area contain pathogens relevant to public health.