Effect of Enriched Rotifer (Brachionus plicstilis) with Probiotic Lactobacilli on Growth, Survival and Resistance Indicators of Western White Shrimp (Litopenaeus vannamei) larvae

Document Type : Research Paper


1 Islamic Azad University

2 Shrimp Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Bushehr, Iran


This study examined the effects of a commercial Lactobacillus probiotic on growth, survival and resistance of western white shrimp (Litopenaeus vannamei) larvae against salinity and formalin stresses in Persian gulf and Oman sea ecology research center. In this experiment, larvae were fed on 6 times a day from mysis I (M1) step to post larvae 5 over 3 treatments with 3 repetitions including a control treatment C (without probiotic) and 2 experimental treatments named A (having probiotic enriched rotifer) and B (having probiotic enriched rotifer and adding probiotic powder directly to the water) in 9 plastic tanks (20-liter) containing 10-l of seawater with concentration of 50 larvae per liter. At the end of this period biometric larvae were studied under salinity and formalin stresses. Results showed that used probiotic bacteria have so significant and positive effects on shrimps' resistance, survival and growth (P < 0.05) that growth and survival rate of larvae fed with A and B treatments are significantly higher than the control group (P < 0.05). the results of the stress tests, also, indicated that probiotic containing treatments (A and B) have significantly higher survival rates in salinity and formalin stresses than control treatment (P < 0.05). The highest rate of survival and resistance was observed in treatment B which was not statistically different from that of treatment A (P > 0.05). According to the results, that using probiotic powder is effective on growth, survival and resistance rate of larval and post larval steps of western white shrimp.