Comparative evaluation of metabolic enzymes activities in different tissues of Pangasianodon hypophthalmus (Sauvage, 1878) fingerlings reared at higher temperature

Document Type : Research Paper


ICAR-Central Institute of Fisheries Education Mumbai – 400 061, India


A 60-days feeding trial was conducted to study the effect of higher temperature (32°C) in comparison to ambient temperature on different metabolic enzyme namely, Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Adenosine triphosphatase (ATPase), Acid Phosphatase (ACP) & Alkaline Phosphatase (ALP) activities in different tissues of Pangasianodon hypophthalmus. AST activity differs significantly (P<0.05) in case of liver, however, temperature do not significantly affect the AST activity in case of muscle (p>0.05). The ALT activity differs significantly (P<0.05) in case of liver and muscle at the different temperature. ATPase activity (at 37°C) in gill do not differ significantly (P>0.05) whereas ATPase activity in the liver at different temperature differ significantly (P<0.05). ACP activity does not vary significantly at different temperature whereas, ALP activity varies significantly at different temperature in liver and intestine tissues. The present study clearly shows that there is a temperature dependent change in the activity of the different metabolic enzyme in different tissue studied in comparison to the control at ambient temperature fed with different energy level diet. The finding of the present study suggests that the metabolic responses are variable at different temperature and different energy level which can affect the growth performances of this new candidate species for aquaculture.