Effects of cortisol treatment and ovaprim treatment on sex steroid hormones, plasma and oocyte cortisol content and ovulation induction in Caspian kutum, "Rutilus frisii (Kamansky, 1901)" broodstocks

Document Type : Research Paper

Authors

1 Agricultur Research, Eduction and Extention Organization, Tehran, Iran. Tehran, Iran.

2 Expert of Fisheries Department, Institute of Technical and Vocational Higher Education, Agriculture Jihad- Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.

3 Associated Professor, Institute of Technical and Vocational Higher Education, Agriculture Jihad- Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.

4 Member of Science, Institute of Technical and Vocational Higher Education, Agriculture Jihad- Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.

Abstract

Caspian kutum fish (Rutilus frisii) wild broodstocks were caught by gillnet during their upstream migration and subjected to short-time confinement stress and their physiological responses have been examined. Thirty fish (average weight of 1217.6 g) were divided into three experimental groups CO (control), OV (ovaprim) and CO+OV (cortisol+ovaprim) and placed into rearing tanks (10 fish tank-1; 2 m3 each) and kept for 10 days. Fish in CO+OV group received a single cortisol injection (20 µg kg-1 B.W.) before being transferred in tank. After 10 days of confinement, all fish in OV and CO+OV groups  were injected by ovaprim (20 µg kg-1 B.W.) to induce maturation and ovulation. Plasma cortisol in CO+OV fish have showed a gradual decrease during confinement and reached to the lowest level after maturation (ovulation) while the highest values for glucose has been found in OV group (p<0.05). Testosterone and 17β-estradiol levels declined significantly in all experimental groups following cortisol treatment and maturation, while no significant difference had been found in the content of plasma progesterone among brood-stocks (p<0.05).The concentration of oocyte cortisol in CO+OV was 2-fold higher than that of CO and OV after maturation. These results indicated that cortisol treatment with subsequent ovaprim injection decreased plasma sex steroids and increased oocyte cortisol content in confined broodstocks but had no effect on oocyte histological characteristics.

 

Keywords

Iranian Journal of Fisheries Sciences
Volume 19, Issue 2
March 2020
Pages 715-725

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