Evaluation of killed vaccine efficacy on hematology parameters and IgM assay of Acipenser stellatus (Pallas, 1771) challenged with Nervous Necrosis Virus (NNV)

Authors

1 Department of Basic Science and Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran.

3 Department of Basic Science and Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Abstract

Vaccination is considered the most effective, environmentally friendly and cost-effective way of protecting commercial finfish species against infectious diseases with high economic impacts. Efficacy of a new inactivated Viral Nervous Necrosis (VNN) vaccine through heat-inactivated nervous necrosis virus (NNV) combined with three types of adjuvants was assessed in 7 g juvenile of Acipenser stellatus (Pallas, 1771) as a laboratory model. Ten groups of 30 juveniles were  divided in 18 aquariums to vaccinate via immersion and injection routes using 4 types of vaccines. Vaccination was performed in two stages one month apart and one month after the second replication, exposure to the live virus was performed. During this period, the mortality rate of immersion and injection groups was 12.9% and 19.8%, respectively, compared to 100% mortality in the control group. Sampling was performed in four stages before the first vaccination in the adaptation period, after the first vaccination, after the second replication, and after exposure to live virus. Blood samples were measured to evaluate changes in blood factors (red blood cell count, white blood cell count, hematocrit and hemoglobin) and immunity factors (immunoglobulin M (IgM). In order to prove the virulence of the viral sample (supernatant) used in exposure, the pathogenicity of VNN virus isolated from infected Golden grey mullet (Chelon auratus) with (TCID50 ) was examined in several guppies. One month after the second repetition of fish, all treatments were exposed to the acute virus sample (supernatant), and after one month, their survival percentage, blood factors and safety were measured.

Keywords

Iranian Journal of Fisheries Sciences
Volume 21, Issue 1
January 2022
Pages 301-315

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