Physicochemical and antioxidant properties of chitosan-coated nanoliposome loaded with bioactive peptides produced from shrimp wastes hydrolysis


1 1. PhD graduate, Department of Processing of Fishery Products, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

2 2. Professor, Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.


The aim of the present study in the first stage is an encapsulation of peptides produced from the hydrolysis of shrimp wastes (by neutrase enzyme) with a combined coating of nanoliposome and chitosan in the form of four treatments (NP or peptide-carrying nanoliposomes, NP-CH-0.05, NP-CH-0.1 and NP-CH-0.5 or peptide-carrying nanoliposomes with a coating of 0.05, 0.1 and 0.5% chitosan). In the next step, the physicochemical and antioxidant properties of the treatments were investigated. The results showed that the average particle size and particle dispersity index in different treatments varies from 228.9±4.85 to 436.7±1.08 nm and 0.389±0.01 to 0.453±0.01, respectively. Zeta potential in nanoliposomes shifted to positive values with increasing chitosan concentration and reached from -62.59±4.36 mV in NP to +56.94±3.71 mV in NP-CH-0.5. The encapsulation efficiency was improved by adding chitosan to the surface of nanoliposomes and the highest amount was related to NP-CH-0.5 treatment (94.12±3.73%). Evaluation of release profiles of treatments in Simulated Gastric Fluid (SGF) and Simulated Intestinal Fluid (SIF) showed that coating of peptides using nanoliposome and chitosan helps to protect the structure of the peptides and reduce their release rate significantly (p<0.05). Chitosan-coated nanoliposomes showed more antioxidant activity than NP treatment. Also, in chitosan-coated treatments, with increasing chitosan concentration, the antioxidant activity of the treatments increased significantly (p<0.05). According to the results, coating bioactive peptides with a combined coating of nanoliposomes and chitosan is a suitable technique to protect and increase the efficiency of peptides.