The Effect of Cryopreservative Extender Developed for Mesopotamian Spiny Eel (Mastacembelus mastacembelus Banks&Solander, 1794) on Sperm Motility and Fertilization After Thawing


1 Department of Fisheries and Aquaculture, Bozova Vocational High School, Harran University 63850, Bozova, Şanlıurfa, Turkey

2 Independent Researcher, Çarşı PTT PK 138 Konya, Turkey


This study aimed to determine the effect of freezing extender developed for Mesopotamian spiny eel (Mastacembelus mastacembelus Banks&Solander, 1794). Semen was collected from eight males weekly by abdominal massage after the hormonal injections of human chorionic gonadotropin (hCG) treatment. Spermatozoa motility and concentration were evaluated on day of collection. The pooled ejaculate was split into 2 equal aliquots and diluted with the ArŞaDo extender and the P1 extenders. Sperm motility of thawing samples was significantly higher in ArŞaDo extender than in P1 extender (42.66±1.17 % vs. 32.96±1.27%, respectively). The fertilization rates in ArŞaDo extender were higher than the rates in P1 extender (82.03±1.94 % vs. 73.43±1.45 %, respectively). In conclusion, the ArŞaDo extender improved the post-thaw semen motility and fertilization rates in Mesopotamian spiny eel males.