Efficiency of dietary black seed (Nigella sativa) essential oil for incisional skin wound healing in goldfish (Carassius auratus)


1 Shahid Bahonar University of Kerman, Kerman, Iran.

2 Department of Food Hygiene and Public Health, Shahid Bahonar University of Kerman, Kerman, Iran.

3 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.

4 Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran.



This study evaluated the healing effect of black seed essential oil (Nigella sativa; NS) dietary supplementation on skin wound in goldfish (Carassius auratus). Some 48 goldfish were divided into 4 groups, including the control and 3 treatment groups in three replications and kept for study period of 7 days. NS essential oil was added to the basal diet of treatment groups at the concentration of 2% for the NS2 group, 4% for the NS4 group, and 6% for the NS6 group. The control group was fed the basal diet without NS during the experimental period. The 5 mm incision made by scalpel on the right flank of all fishes below the dorsal fin above the lateral line. The fish fed three times a day with assigned diet for a week. Histological investigations approved the effects of NS essential oil on skin wound healing. Diet with 6% concentration of NS6 revealed the improved healing outcomes such as re-epithelialization, muscle regeneration and scale formation rather than NS2 and NS4 groups.