Department of Animal Processing, Animal Science Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Yas Aquatic Protein Limited, Guilan Science and Technology Park (GSTP), Ministry of Science, Research and Technology, Rasht, Iran.
Developing aqua/ animal feeds from underutilized fish and agricultural by-products using hydrolyzed technology is an excellent way to provide proteins for aquaculture/ animal farming. This study reports development of a new protein feed supplement, based on fish protein hydrolysate and agricultural residues, on a pilot scale and investigates physicochemical properties, microbial quality, and shelf-stability of the product. The production conditions were based on lab-scale experiences. Accordingly, fish protein hydrolyzed solution containing 16.58% crude protein, 63.35% moisture, and 15.52% fat was mixed with agricultural by-products, i.e. sesame meal, rice bran, and soybean meal as fillers (60:40 w/w) and dried at 40°C for 6 h. The dried mixture was grounded after cooling, then packed in sealed plastic bags, and stored for 180 days at ambient temperature. The protein feed supplement contained 58.23% crude protein, 16.18% fat, 8.85% moisture, 9.10% ash, 2.6% fiber, 1.489% calcium, 0.89% phosphorous, and 5.34% carbohydrates of dry weight. Total essential and non-essential amino acids were 19.41g/ 100g and 20.36 g/ 100g of dry weight respectively. The protein feed supplement contained significant essential fatty acids. Levels of pH and TVB-N of the protein feed supplement increased to 5.30 and 65.20 mg/100g at the end of the study. After 180 days, there were no yeast and mold in the samples and it was still pathogen-free.