Histology of the inflammatory response of carp (Cyprinus carpio L.) to fungus, Aphanomyces invadans, infection

Authors

1 Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran.

3 Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran.

10.22092/ijfs.2023.129755

Abstract

The present investigation was carried out to study the inflammatory response of carp (Cyprinus carpio L.) to the fungus Aphanomyces invadans infection which is known as a causative agent of Epizootic Ulcerative Syndrome (EUS). Thirty two carps with an average length of 13cm (±2cm) were injected intramuscularly with 0.1 ml of the Aphanomyces invadans spore suspension. Two injected fish were sacrificed by an overdose of 10% benzocaine, at 6 hours, 1, 2, 3, 4, 5, 6, 7, 10, 14, 18, 22, 28, 35 and 42 days after inoculation. Blocks of tissue were fixed in 10% buffered formalin then processed and stained with haematoxylin and eosin, Grocott and periodic Acid Schiff (PAS) for histologic examinations. A chronic inflammatory response, consisted of cellular infiltration, vascularization, fibrosis and granulomata formation, occurred after inoculation of spores of Aphanomyces invadans at water temperature of 27°C (± 1.5°C). Macrophages which infiltrated the lesion area in early stages took on an epithelioid configuration at 3 days post injection. In addition to classical Langhans and foreign body type of giant cells which were observed 3 days after infection, also intermediate type was seen in the lesion area. With continuing healing, giant cells reduced in number by 14 days and disappeared at 18 days after injection. Muscle regeneration started at 3 days and the defect area was filled by new muscle bundles by 14 days. Fibroplasia, along with vascularization, started at 3 days and well developed granulomata formed by 10 days and then fully matured granulomata which filled the whole defect area and were surrounded by the normal muscle bundles were observed by 18 days post injection. It is apparent from the results of the present study that healthy carp resist Aphanomyces invadans infection at high temperatures (27°C) by an active defence mechanism and employing a strong inflammatory response.

Keywords

Iranian Journal of Fisheries Sciences
Volume 22, Issue 4
July 2023
Pages 771-789

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