Effects of temperature stress on genome-wide DNA methylation levels in the sea cucumber, Apostichopus japonicus

Authors

Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, 116023, China

10.22092/ijfs.2024.131219

Abstract

Sea cucumber Apostichopus japonicus lives in the lower temperature of the seawater and will enter the state of aestivation when the temperature is high. In recent years, the continuous high temperatures in summer have brought great loss to the production of sea cucumber in north China. To study the epigenetic regulating mechanism of A. japonicus and guide the temperature-controlled culture, the methylation level of A. japonicus genome was detected. Breeding imitation A. japonicus under different temperature conditions for a period of time, the difference of methylation level between the set temperature group and the control group was detected and verified. The methylation levels of A. japonicus in different temperature groups were detected. The methylation sites ranged from 84,766 to 96,225, and the sequencing depth was between 42.33 and 153.21. Under the temperature stress, more than 60% of the differentiated methylated genes in different groups were down-regulated, the HBW group had the most differentiated methylated genes (1,405), and the SBW group had the least differentiated methylated genes (643). Gene function classification and enrichment analysis were carried out, and a total of 3,512 differential genes were found, mostly related to immunity and metabolism. The results showed that temperature can change the gene methylation level of A. japonicus, affecting its expression level, and ultimately changing the metabolic and immune processes of A. japonicus.

Keywords

Iranian Journal of Fisheries Sciences
Volume 23, Issue 3
May 2024
Pages 437-456

Files

Share

How to cite

Statistics