Effect of an encapsulated blend of nano-selenium and vitamins C and E on growth performance, blood, immunity, and oxidative indices in Huso huso

Authors

1 International Sturgeon Research Institute, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran.

2 Department of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran. PO Box: 163

3 Department of Fisheries, Faculty of Animal Science and Fisheries, University of Agriculture and Natural Resources, Sari, Iran. PO Box: 578

10.22092/ijfs.2025.134040

Abstract

A 10-week feeding trial was conducted to investigate the effect of dietary supplementation with encapsulated Nano-selenium (NanoSe) and vitamins C and E at varying dosages (Control: free from the supplementations; T1: 0.1, 30, and 100 mg/kg; T2: 0.2, 60, and 200 mg/kg; T3: 0.3, 90, and 300 mg/kg, respectively) on the growth performance, hematology, immunity and antioxidant indices of juvenile Huso huso. The fish, with an average initial weight of 78.54±0.90 g, were stocked in 12 fiberglass tanks and fed with the respective diets for 10 weeks. The fish fed with diets T1 and T2 exhibited significantly higher final weight, feed conversion ratio, and protein efficiency ratio compared to the fish fed with T3 and the control diets (p<0.05). Additionally, the white blood cell count (WBC), hemoglobin (Hb), and neutrophil levels increased in the fish fed with T1 and T2 diets compared to the control group (p<0.05). The alternate complement activity (ACH50) was higher in T2 and T3 groups compared to the control group (p>0.05). The serum lysozyme activity showed no significant difference across groups T1, T2, and T3 (p>0.05). The result showed that IgM levels in the fish fed with the supplemented diets (T1-T3) showed no significant difference compared to the control group (p>0.05). The total lipids and albumin in T1 and T2, total protein in T2, and cholesterol in all treated-groups were increased compared to the control group (p>0.05). The fish fed with supplemented diets had significantly lower levels of serum cortisol and glucose compared to the control group (p<0.05). Supplementation resulted in improved antioxidant status, as demonstrated by increased superoxide dismutase levels in the T2 group and decreased malondialdehyde levels in all supplemented groups (T1, T2, and T3) compared to the control group (p<0.05). This study suggests that dietary supplementation with a moderate mixture of NanoSe (0.2 mg/kg) and vitamins C (60 mg/kg) and E (200 mg/kg) positively promotes growth, improves some blood indices, stimulates the immune system, and reduces oxidative stress in juvenile Huso huso.

Keywords

Iranian Journal of Fisheries Sciences
Volume 24, Issue 4
July 2025
Pages 1015-1040

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