Changes in miR-462 gene expression of rainbow trout in response to cerebral hemorrhage caused by viral hemorrhagic septicemia (VHS)

Authors

1 Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran

2 Department of Physiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran

3 Agricultural Research and Education Organization of Iran, Tehran, Iran

10.22092/ijfs.2025.134260

Abstract

This study investigates the expression of miR-462 in rainbow trout (Oncorhynchus mykiss) during viral hemorrhagic septicemia (VHS) infection to understand its role in disease pathogenesis and immune defense. MicroRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression at the post-transcriptional level. VHS is a highly contagious and economically significant viral disease affecting farmed rainbow trout. In this study, 300 rainbow trout were divided into three groups: a negative control group (uninfected), a positive control group (infected without intervention), and an experimental group (infected via intraperitoneal injection with VHS virus). Brain tissue samples were collected over 14 days, and miR-462 expression levels were quantified using molecular techniques. The results showed a significant upregulation of miR-462 in the experimental group, with a 3.5-fold increase compared to controls (p<0.05), indicating its involvement in the immune response to VHS infection. These findings suggest that miR-462 plays a role in the host's response to viral infection. The study highlights the need for further investigation into the specific pathways regulated by miR-462 and its potential as a biomarker for VHS. Limitations include sample size variability, and future studies should explore the functional implications of miR-462 upregulation and its utility in disease management strategies.

Keywords

Iranian Journal of Fisheries Sciences
Volume 24, Issue 5
September 2025
Pages 1175-1186

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