Anti-Alzheimer and antioxidant activities of bacteria isolated from the Persian Gulf sponges

Authors

1 Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran

2 Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Bandar Abbas, Iran

10.22092/ijfs.2026.135842

Abstract

Acetylcholinesterase (AChE) inhibitors are vital in the management of Alzheimer’s disease. This study aimed to identify bacteria isolated from sponge samples collected near Hengam Island and evaluate their acetylcholinesterase inhibitory activity. Sponge samples of Halicona sp. and Axinella sp. were collected by scuba diving. A total of 131 bacterial strains were isolated using Marine Zobell agar medium. Bacillus and Vibrio species were the most frequently identified bacteria from the sponge samples. Analysis of the extracted metabolites revealed that four isolates could inhibit acetylcholinesterase activity, with IC50 values between 203.60 and 385.62 μg/ml. According to the MTT cell proliferation assay, HH 151 extract exhibited cytotoxic effects on the HUVEC cell line. The AChE inhibitory extracts also showed DPPH radical scavenging activity between 78.12 μg/mL and 237.54 μg/mL. The 16S rRNA sequence analysis showed about 99 to 100% homology between HH 127, HH 151, HA 315, and HA 338 strains with Vibrio azureus, Streptomyces nigrescens, Bacillus pumilus, and Bacillus subtilis species. These findings provided new candidates of the acetylcholinesterase inhibitors from sponge-associated bacteria for in vivo studies.

Keywords

Iranian Journal of Fisheries Sciences
Volume 25, Issue 4
July 2026
Pages 617-635

Files

Share

How to cite

Statistics