Effects of short-time alkaline pretreatment on growth and photosynthesis efficiency of endemic cyanobacterium Fischerella sp. FS 18.

Document Type : Research Paper

Authors

1 1- Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran.

2 2- Department of Chimistry, Gorgan Branch, Islamic Azad University, Gorgan, Iran.

3 3- Young Researchers and Elite Club, Gorgan Branch, Islamic Azad University, Gorgan, Iran.

Abstract

Alkaline pH is one of the most important problems of our aquatic habitat. We used Stigonematalean native cyanobacterium Fischerella sp. FS 18 as our model strain, andstudied it under different alkaline pHs (7, 9 and rarely 11) under two different – short and long- time treatments (24 and 96 hours after inoculation). Spectroscopic results showed that both alkalinity and time affected growth rate, phycobilisome and chlorophyll production. Response surface plot analysis of distribution showed that the pH borders between 8.5 to  9 would be critical at 24 hours after inoculation reaching to the highest rates of phycobilisomes. Spectrofluorimetric analysis showed that the highest photosystem I/ photosystem II may be seen at 24 hours at pH9. Photosynthesis- Irradiance curves showed that the highest rate of maximum photosynthesis belonged to pH9 in the short time treatment (24 hours). Increasing the time (96 hours) decreased the maximum amount of oxygen liberation significantly. Moving from the optimum conditions (even slightly) caused a sharp decline in the amount of alpha. The slope of decline was steep until near neutral and longtime condition.  Decreasing the time, and at the same time increasing alkalinity (alkaline pH) caused higher activity of photosystems especially photosystem I which lead to higher reductant production and cyclic electron flow operation. Distinct borders of pH (8.5-9) at 24 hours caused considerably high growth and matter production. This was naturally true for phycocyanin, phycoerythrin and the other economically important matters.  

Keywords

Iranian Journal of Fisheries Sciences
Volume 19, Issue 6
November 2020
Pages 2865-2876

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