Molecular identification of Vibrio species in waste water urban effluent of the Persian Gulf: Implications for shrimp industry

Document Type : Research Paper


1 azad university shiraz, science, agiculture and new thechnology college, microbiology department

2 shrimp reseach center, iranian fisherie science research institute, agricultural research education and extension organization

3 iranian shrimp research institiute


Vibriosis is one of the major diseases which cause problems in the aquaculture industry. The disease is a bacterial infection responsible for the mortality of breeding shrimp around the world. This study aimed to isolate and identify V. alginolyticus contaminated shrimp samples in Bushehr city, Southwest Iran. Shrimp samples were collected from Persian Gulf waste water. Identification of bacterial isolates was performed by phenotypic and biochemical and molecular tests (PCR and sequencing) by using forward primer (5-GAGTTTGATCCTGGCTCAG-3) and reverese primer (5-ACGGGCGGTG+GTRC-3). Antibiotic susceptibility profile of isolates was detected. isolate of V. alginolyticus was detected with resistance to ampicillin, but susceptible to cefotaxime, ceftazidime, chloramphenicol, ciprofloxacin, gentamicin, and imipenem and tetracycline disks.  V. alginolyticus is participated in shrimp contamination which lead to food destroy and also human impairments. Key words: Vibrio alginolyticus, primer, Vibriosis, 16srRNA sequencing