Molecular genetic divergence of five genus of cypriniform fishes in Iran assessed by DNA barcoding

Document Type : Research Paper


1 Shahid Motahary Cold-water Fishes Genetic and Breeding Research Center,Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Yasouj, Iran

2 Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

3 Center for Integrative Ecology, School of Life and Environmental Sciences, Faculty of Science, Engineering and Built Environment, Deakin University, Victoria 3280, Australia


The present study represents a comprehensive molecular assessment of some family of freshwater fishes from Iran. We analyzed cytochrome oxidase I (COI) sequences for five genus of cypriniform fishes from Iran. The present investigation provides data on genetic structure of some species of Nemachilidae including Paraschistura bampurensis, Oxynoemacheilus kiabii and Turcinemacheilus saadii and leuciscine cyprinids Alburnoides bipunctatus and Alburnus  alburnus from inland waters of Iran. The DNA sequences of the mitochondrial COI gene in all fishes were amplified, and the resulting sequences were compared to entries in GenBank by using the Basic Local Alignment Search Tool for nucleotide data. Individuals were assigned to groups using COI gene sequence divergence analysis was determined using Kimura 2-parameter distances. Results revealed two major clusters which were inconsistent were characterized by mediocre genetic divergence (mean 0.8%). The sequences of P. bampurensis showed that specimens collected from four Rivers (Beshar, Khersan, Shapour and Fahlian, south-west of Iran), with 1.89% for Nemacheilian loaches and 0.8%) for two leuciscine cyprinids within-species Kimura two parameter distance, shared haplotype in Neighbor-joining (NJ) tree. Some clusters showed haplotype sharing, or low levels of divergence between species, hindering reliable identification. We discussed the importance of further DNA barcode studies for native and invasive cyprinid species and subsequent submission to GenBank databases for more reliable species match and inference.