Frequency and antimicrobial resistance of Yersinia enterocolitica isolated from fish meat caught in Anzali Wetland

Authors

1 Department of Heath and Food Quality Control, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

2 Department of Environment, Faculty of Agriculture and Natural Resources, Isfahan Khorasgan Branch, Islamic Azad University, Isfahan, Iran

Abstract

Yersinia enterocolitica is the cause of yersiniosis in humans and its symptoms range from mild diarrhea to mesenteric lymphadenitis that may lead to appendicitis. In this study, the frequency and antibiotic resistance of Yersinia enterocolitica isolated from fish caught in Anzali wetland were investigated. Sampling was carried out for one year in four seasons from the autumn 2018 to the summer 2019 from four geographical directions: east, west, north and south, from different wetland levels. A total number of 91 fish were sampled from the body of these fish. Microbiological tests including biochemical tests, specific culture, and PCR were performed. In the specific culture method, 20 samples (21.97%) were contaminated with Yersinia enterocolitica. For the final confirmation of PCR, 20 samples were isolated from 14 samples (15.4%) containing specific culture method (6 Carp, 3 Anchovy, 0 Bream, 4 Pike Perch and 1 Pike) were infected with Yersinia enterocolitica. The chi-square test showed that there was no statistically significant difference between the various seasons (p >0.05), while there was a significant difference between fish species (p <0.05). Isolates from Yersinia enterocolitica using an antibiogram test of 10 antibiotics, the most susceptible isolates, Imipenem (92.8%), Kanamycin and Gentamicin (85.7%), Ceftazidime (78.57%). Cotrimoxazole and Nalidixic acid (71.4%) were the most resistant isolates to Ampicillin (64.28%), respectively. The results showed high levels of contamination of Yersinia enterocolitica, and indicative of potential dangers of Yersinia enterocolitica pathogenicity, especially in immunocompromised and susceptible individuals. Further studies on the pathogenicity of Yersinia enterocolitica in fish within the Anzali wetland and preventing sewage from entering the wetland are suggested.

Keywords

Iranian Journal of Fisheries Sciences
Volume 23, Issue 5
September 2024
Pages 771-781

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